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KMID : 0380020050200040260
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Korean Journal of Biotechnology and Bioengineering
2005 Volume.20 No. 4 p.260 ~ p.265
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Effect of Functional Monomer on Retention Factor of Chiral Racemate
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Kim Eun-Cheol
Row Kyung-Ho
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Abstract
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In this work, molecular imprinted polymers (MIPs) using the template of the N-CBZ (carbobenzyloxy)-L-phenylalanine, MAA and 4-VPY as a monomer, EGDMA as a crosslinker and AIBN as an initiator were considered. The prepared polymer particles were packed into a chromatographic column . The chromatographic characteristics of the retention on the MIP were experimented with acetonitrile as a mobile phase at the flow rate of mobile phase, 0.5 ml/min. The retention factors and resolutions of chiral racemate of the N-CBZ-D, L-phenylalanine were measured. The results showed that the retention factor and resolution by the two co-monomer imprinting polymer were higher than the single monomer imprinting polymers, which indicated an increase in the affinity of the MIP with the sample as a result of the cooperation effect of the binding sites.
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KEYWORD
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Molecular imprinted polymer, co-monomer, hydrogen binding, chiral racemate, retention factor
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